Diversity and characterization of endophytic fungi. The extracts were screened for anticancer assay against human colon cancer cell lines ht29. Kumar kalavathi murugan, chandrappa chinna poojari, channabasava ryavalad, ramachandra yarappa lakshmikan, padmalatha rai satwadi, ravishankar rai vittal and govindappa melappa, antidiabetic activity of endophytic fungi, penicillium species of tabebuia argentea. May 14, 2014 anticancer activity of new depsipeptide compound isolated from an endophytic fungus. Diversity and anticancer activity of endophytic fungi associated with the medicinal plant saraca asoca article pdf available august 2015 with 422 reads how we measure reads. Mechanism and anticancer activity of the metabolites of an endophytic fungi from eucommia ulmoides oliv volume. Classification of endophytic fungi strains the 30 endophytic fungi strains with antimicrobial activity against at least three tested pathogens, which also showed antitumor activity against at least one test human tumor cell line, which were called highly active strains.
Endophytic fungi are microorganisms which spend the whole or part of their lifecycle residing symbiotically within the healthy tissues of host plants, interandor. Biological activity of endophytic fungi from the roots of. Endophytic fungi are a group of fungi that colonize living and internal tissues of plants without causing any immediate, overt negative effects. It is plausible that compounds from the secondary metabolism of endophytic microorganisms may contribute to the claimed anticancer activities of fermented noni fruit juice in humans, either via immunological or, as yet, unidentified mechanisms in vivo. Cytotoxic and antimicrobial activities of ethyl acetate extract. Two species of endophytic fungi efb01 and efb02 were isolated from leaves of barringtonia acutangula, a medicinal plant and secondary metabolite extraction was performed using organic solvents. Anticancer activity of new depsipeptide compound isolated. Rhizophora mucronata is an important ecosystem entity of the malaysian mangrove forest. Production of anticancer enzyme asparaginase from endophytic. Isolation and identification of endophytic fungi from. Mechanism and anticancer activity of the metabolites of an.
The uniqueness of the endophytic community of fungi is stressed as a promising source of novel compounds with anticancer activity, or as an alternative source. Bioactivity assessment of endophytic fungi associated with centella asiatica and murraya koengii. Interest in endophytes as natural sources for new medicines was inspired by the discovery of paclitaxelproducing endophytic fungi. A total of 320 fungal isolates were obtained, and 66 phylotypes comprising 25 genera were identified. The experimental design used was a completely randomized factorial design with.
This study was conducted to isolate lasparaginaseproducing endophytic fungi from medicinal plants of family asteraceae. The culture pm0509732 was isolated as endophytic fungi from the leaves of. Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf annona muricata l. To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi. The uniqueness of the endophytic community of fungi is stressed as a promising source of novel compounds with anticancer activity, or as an alternative source of compounds originally isolated from higher plants. Antimicrobial activity of crude extracts of endophytic fungi isolated from medicinal plant trichilia elegans a. Abstract endophytes are microorganisms which inhabit inside plants. A number of plant endophytic fungi have been shown to have anticancer activity 12. Anticancer activity of morinda citrifolia noni fruit. Anticancer activity and mechanism investigation of. In addition, it describes how some biotechnological processes can help in the discovery of bioactive compounds, especially with anticancer activity. Pdf bioprospecting endophytic fungus colletotrichum sp. This study aimed to investigate the in vitro toxicity activity of ethyl acetate extracts of endophytic fungi isolated from soursop leaves annona muricata l. An endophytic fungi is a fungal microorganism, which spends the whole or.
Antibacterial and antioxidant activities of endophytic fungi. Antifungal and antiproliferative activities of endophytic. Various compounds including camptothecin and several analogues 79, vincristine and podophyllotoxin 10,11, with anticancer activity have been isolated since the discovery of fungal derived paclitaxel 2. Production of bioactive secondary metabolites from endophytic.
The designation anticancer is based on the assessment of the authors of the paper of. Endophytic fungi as alternative and reliable sources for. Of the total number of substances isolated from endophytic fungi, 57% are new or analogues of known compounds ravindra et al. Anticancer and antifungal compounds from aspergillus. Purification, crystallization and anticancer activity evaluation of the. So, for example, taxol and related compounds, a potent anticancer medicine, taxane prototype, which was first obtained by. Inouye college of pharmacy, university of hawaii at hilo, hilo, hi, usa. In total, 172 endophytic fungi were isolated in our survey. Sclerotiorin is an orange yellow colored pigment belonging to. In addition, it describes how some biotechnological processes can help in the discovery of bioactive. Further sclerotiorin was isolated from the active fungal extract by activity guided isolation. Endophytic fungi from the chinese medicinal plant actinidia macrosperma were isolated and identified for the first time.
The present study was intended to test the anticancer activity in vitro of ethyl acetate extracts of soursop leaf endophytic fungi in the search for antibreast cancer. Cytotoxic and antimicrobial activities of endophytic fungi. Dec 10, 2015 the role of endophytic fungi in the anticancer activity of morinda citrifolia linn. Asparaginase is an important anticancer enzyme, endophytic fungi eurotium sp.
Characterization of cytotoxic compound from mangrove derived. The role of endophytic fungi in the anticancer activity of morinda citrifolia linn. The products were obtained from the endophytic fungi. Endophytes, which reside in plant tissues, have the potential to produce novel metabolites with immense benefits for health industry. Endophytic fungi are defined as fungi that live asymptomatically within the tissues of higher plants. In the present work, we isolated, identified and characterized, a total of 78 fungal isolates harboring inside the leaf tissues. Anticancer activities of endophytic fungi isolated from. The present study was aimed at evaluating the anticancer activity of the crystallized compound alternariol methyl ether ame against hepatocellular carcinoma hcc both in vitro and in vivo from an endophytic fungus residing in the medicinal plant vitex negundo. Anticancer activity test of ethyl acetate extract of. Secondary metabolites from endophytic fungi have been recognized as potential source of anticancer compounds 36. This study investigated the anticancer activity of polysaccharides produced by an endophytic fungus chaetomium globosum cgmcc 6882, which was isolated from g. Potensi ekstrak kapang endofit asal rimpang kunyit sebagai antimalaria dan antioksidan. Lasparaginase is an important anticancer enzyme that is used in the first line treatment of acute lymphoblastic leukemia. Anticancer activity of polysaccharides produced from glycerol.
Antibacterial and antioxidant activities of endophytic. Production of bioactive secondary metabolites from. Endophytic fungi are diverse group of fungi which are symbiotically associated with plants. It also considersand their medicinal applications especially in the production of anticancer, antimicrobial, antioxidant, and. Noni noni yougen wu, 1, 2 sisay girmay, 1 vitor martins da silva, 1 brian perry, 3, 4 xinwen hu, 2 and ghee t. Key laboratory for space bioscience and space biotechnology, school of life sciences, northwestern polytechnical university. Introduction endophytic fungi have been studied since 1898 1, but only a limited number of endophytic fungi were reported in the years 18981980s 2. Anticancer potentials of secondary metabolites from endophytes of. Endophytic fungi from medicinal plants wiyakrutta et al. Anticancer potentials of secondary metabolites from endophytes of barringtonia acutangula and its molecular characterization p. This research was carried out to evaluate the antibacterial and antioxidant activity of 40 endophytic fungi isolated from 10 species of medicinal plants collected.
Research article open access isolation, purification and. Tan 1 department of pharmaceutical sciences, daniel k. Diversity and antifungal activity of endophytic fungi. Preliminary screening of extracts or compounds using cytotoxicity assays allows the rapid identification of compounds with anticancer activity. Endophytic fungi with antimicrobial, anticancer and anti. Anticancer properties are generally associated with the cytotoxicity of the secondary metabolites present in the endophytic fungi 48.
Research article the role of endophytic fungi in the anticancer activity of morinda citrifolia linn. Here we report in vitro study on anticancer activity of ethyl acetate extract of endophytic fungi isolated from soursop leaf a. Pdf anticancer activity test of ethyl acetate extract of. The diversity, antimicrobial and anticancer activity of. Endophytic microbes usually create symbiotic interactions with plant tissues. Endophytic fungi residing in medicinal plants have the. Article pdf available in asian pacific journal of tropical medicine 106. Endophytic fungi have been shown to produce several pharmacologically important compounds such as antimycotics steroid 22triene3bol 5, anticancer cajanol 6, podophyllotoxin and kaempferol 7, antiinflammatory ergoflavin 8, antioxidant lectin 9, insecticidal heptelidic acid 10, immunosuppressive sydoxanthone a, b 11 and cytotoxic radicicol 12. Endophytic fungi were isolated from the bacopa monnieri. The diversity and biological activities of the endophytic fungi associated with the brazilian medicinal plant stryphnodendron adstringens were studied. Anticancer and antibacterial secondary metabolites from.
Endophytes were isolated from surfacesterilised leaf tissue, grown as pure cultures. The classification of the highly active endophytic fungi. A total of 81 thai medicinal plant species collected from forests in four geographical regions of thailand were examined for the presence of endophytic fungi with biological activity. Purification, crystallization and anticancer activity. Request pdf isolation, identification and anticancer activity of an endophytic fungi from juglans mandshurica the endophytic fungus named fsn006 was isolated from the inner bark of juglans. We can conclude that the endophytic fungi are a novel and important microbial resource for producing bioactive compounds and have attracted attention of many researchers on their theoretical study as well as their potential applications. Diversity and anticancer activity of endophytic fungi. Due to their antifungal and anticancer activity, endophytic fungi have attracted increasing attention, and are considered as promising biological control. Diversity and antioxidant activity of culturable endophytic fungi from alpine plants of rhodiola crenulata r. Odukoyaa adepartment of pharmacognosy, faculty of pharmacy, university of lagos, college of medicine campus, lagos.
Dicotyledonous plants such as soursop are predicted to have endophytic microbes which are a potential medicinal sources. Diversity and characterization of endophytic fungi isolated. Inflammatory and anticancer activity of ergoflavin. Antitumor potential of seaweed derivedendophytic fungi. Metabolites of endophytic fungi from australian native. Endophyte fungi can produce secondary metabolite which can be used as antioxidant, anticancer and antimicobes compound.
Cytotoxic and antimicrobial activities of endophytic fungi isolated from bacopa monnieri l. Antitumor and antifungal activities in endophytic fungi. Endophytic fungialternative sources of cytotoxic compounds. Research article the role of endophytic fungi in the. Anticancer activity of polysaccharides produced from. Alkaloids are naturally occurring chemical compounds that contain nitrogen atoms.
Pdf anticancer compounds derived from fungal endophytes. Diversity and anticancer activity of endophytic fungi associated with the medicinal plant saraca asoca jinu mv 1 and jayabaskaran c1 1 department of biochemistry, indian institute of science, bangalore, india, email id. This taxol has strong cytotoxic activity towards bt 220, h116, int 407, hl 251 and hlk 210 human cancer. Vanmathi selvi department of microbiology, sri akilandeswari womens college, vandavasi. Anticancer activity test of ethyl acetate extract of endophytic fungi. Five different taxa were represented by 11 isolates, and six isolates were grouped. Antimicrobial and antitumor activity and diversity of. Pdf diversity and anticancer activity of endophytic. Since the species grows in a harsh environment, any organism that is isolated from this species would be of huge interest due to its potential in having novel bioactive compounds. Isolation and identification of lasparaginaseproducing. Extracts from liquid cultures were tested for cytotoxicity against a number of. Isolation, identification and anticancer activity of an.
Antitumor activity was studied by the mtt assay and antifungal activity was determined by observing fungal growth inhibition. Novel natural compounds from endophytic fungi with anticancer. Endophytic fungi are a group of fungi which grow inside the plant tissues without causing negative symptoms to the host plant and are able to produce biologically active substances. Of 582 pure isolates obtained, 360 morphologically distinct fungi were selected for cultivation on malt czapek broth and yeast extract sucrose broth, from which extracts were tested for biological activity. Yiwen tao, yongcheng lin, zhigang she, minting lin, pinxian chen, jianye zhang. Antimicrobial and anticancer activities of endophytic fungi from. The choice of appropriate solvent could improve extraction efficiency. Gond,a andrea stierleb and donald stierleb received 1st february 2011 doi. The role of endophytic fungi in the anticancer activity of. Seven healthy medicinal plants from family asteraceae were selected for the isolation of endophytic fungi using standard surface sterilization techniques. The secondary metabolites isolated from endophytes displayed a broad spectrum of pharmacological properties including anticancer, antiviral. A total of 81 thai medicinal plant species collected from forests were examined for the presence of endophytic fungi with biological activity. The properties of endophytic fungi provide a new approach for the production of bioactive compounds through industrial fermentation.
The anticancer properties of several secondary metabolites from endophytes have. Antimicrobial activity of crude extracts of endophytic. Anticancer activity is generally associated with the cytotoxicity of the compounds present in the endophytic fungi. Potential of the endophytic fungus phialocephala fortinii rac56 found in rhodiola plants to produce salidroside and ptyrosol. Fermentation broths of 172 endophytic fungi were tested for. Qi li, yan zhang, junling shi, yilin wang, haobin zhao, dongyan shao, qingsheng huang, hui yang and mingliang jin affiliation. We hypothesize that the fungal endophytes of noni may possibly play a role in its overall pharmacological repertoire, especially since the perceived efficacy of the fruit in ethnomedicinal use is associated with the fermented juice. These are ubiquitous and occur within all known plant species. Isolation of anticancer and antitrypanosome secondary.
Medicinal plants are known to harbour endophytic fungi that are believed to be. Fungal endophytes establish stable symbiotic association with their plant host by commensalistic, parasitic, or mutualistic means without causing immediate overt effects. Novel natural compounds from endophytic fungi with. Antidiabetic activity of endophytic fungi, penicillium. Alkaloids, be it from plants or their endophytic fungi, have a wide range of biological activities, some of which have been studied as anticancer components. The culture pm0509732 was isolated as endophytic fungi from the leaves of pongamia pinnata l. Noni yougenwu, 1,2 sisaygirmay, 1 vitormartinsdasilva, 1 brianperry, 3,4 xinwenhu, 2 andgheet. The amount of taxol produced by this endophytic fungus, colletotrichum gloeosporioides strain jgc9 was about 163. This was the first study to evaluate their cytotoxic and antitumour activities against brine shrimp and five types of tumour cells, respectively. Anticancer activity of new depsipeptide compound isolated from an endophytic fungus.
Anticancer compounds derived from fungal endophytes. Scientific studies have investigated the anticancer activity of several. Extract obtained from endophytic fungus, efb01 showed. Anticancer activity of new depsipeptide compound isolated from an. The future of discovering anticancer agents from endophytic fungi is undoubtedly bright. The secondary metabolite and bioactive compounds from endophytic fungi are screened for their antiproliferative activity against different cancer cell lines please refer tables 46 for examples. Anticancer potentials of secondary metabolites from. Qi li, yan zhang, junling shi, yilin wang, haobin zhao, dongyan shao, qingsheng huang, hui yang and mingliang jin. Bioprospecting endophytic fungus colletotrichum sp. It also considersand their medicinal applications especially in the production of anticancer, antimicrobial, antioxidant, and antiviral compounds. Jul 25, 2018 rhizophora mucronata is an important ecosystem entity of the malaysian mangrove forest. Anticancer activity of sclerotiorin, isolated from an. The foremost goal of this study is to explore the role of endophytederived secondary metabolites in the purported anticancer properties of noni. Anticancer activity and mechanism investigation of beauvericin isolated from secondary metabolites of the mangrove endophytic fungi.